Difference between revisions of "Describe cellular communication systems used"
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RBS | RBS | ||
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| + | == Lux cell signaling system == | ||
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| + | '''Responsible:''' Andrew Gordon and Pallavi Penumetcha | ||
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| + | '''Signal molecule:''' ''N''-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules | ||
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| + | '''Bacterial species:''' discovered in ''Vibrio fischeri'' known to work in ''E. coli'' | ||
| + | |||
| + | '''Receiver protein:''' LuxR protein receives signal from AHL; also has some control over transciption of luciferase | ||
| + | |||
| + | '''Signal molecule synthase:''' LuxI; also has some control over transciption of luciferase | ||
| + | |||
| + | '''Additional Information:''' "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration | ||
| + | |||
| + | [[Image:800px-Luxrreceiverschematic.png]] | ||
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| + | '''Resources''' | ||
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| + | [http://partsregistry.org/Lux Lux Operon Pathway] | ||
| + | |||
| + | [http://partsregistry.org/AHL AHL signaling molecules by species; some are specific to gram pos but may affect gram negs] | ||
| + | |||
| + | [http://www.horizonpress.com/jmmb/v1/v1n1/03.pdf Reducing Crosstalk in Lux System] | ||
| + | |||
| + | '''References''' | ||
| + | |||
| + | [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522112 Quorum Quenching to control Lux Pathway] | ||
| + | |||
| + | [http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html] A Synthetic multicellular system for programmed pattern formation | ||
| + | |||
| + | '''Parts Needed''': | ||
| + | |||
| + | LuxR + pro/term | ||
| + | |||
| + | RBS | ||
| + | |||
| + | LuxI + pro/term | ||
| + | |||
| + | LuxI sender | ||
Revision as of 14:50, 28 October 2008
Las/Rhl cell signaling system
Responsible: Robert Cool, Alicia Allen, and Erin Feeney
Las System
Signal Molecule: An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)
Bacterial species: Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)
Receiver protein: LasR
Effect of binding: TXN activation of virulence genes, lasA, lasB, apr, toxR
Synthase: LasI enzyme
Target Genes: lasI, lasA, lasB, apr, toxR
Regulation: unknown
References
"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"
JP Pearson, EC Pesci and BH Iglewski [1]
"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"
Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci 2
Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa
Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden 3
Pending: [2]
Rhl System
Signal Molecule: An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)
Bacterial species: Pseudomonas aeruginosa, gram(-)
Receiver Protein: Rhl R
Effect of Binding: activation of Rhamnosyl Transferase, then making RL (rhamnolipid)
Synthase: RhlA and RhlB
Target Genes: pqsABCDE and phnAB
Regulation: unknown
References
background information on Las and Rhl
Parts Needed:
LasR + pro/term
RhlR + pro/term
RhlI + pro/term
pqsABCDE + pro/term
pqsR
pqsH + pro/term
phnAB
LasI
RBS
Lux cell signaling system
Responsible: Andrew Gordon and Pallavi Penumetcha
Signal molecule: N-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules
Bacterial species: discovered in Vibrio fischeri known to work in E. coli
Receiver protein: LuxR protein receives signal from AHL; also has some control over transciption of luciferase
Signal molecule synthase: LuxI; also has some control over transciption of luciferase
Additional Information: "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration
Resources
AHL signaling molecules by species; some are specific to gram pos but may affect gram negs
Reducing Crosstalk in Lux System
References
Quorum Quenching to control Lux Pathway
[3] A Synthetic multicellular system for programmed pattern formation
Parts Needed:
LuxR + pro/term
RBS
LuxI + pro/term
LuxI sender
