Difference between revisions of "Suppressor Logic Toolkit and Quasi-Hash Function"
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prpB 2-methylisocitrate lyase in E.coli MG1655 [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=944990&ordinalpos=3&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum] | prpB 2-methylisocitrate lyase in E.coli MG1655 [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=944990&ordinalpos=3&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum] | ||
Revision as of 15:44, 21 May 2009
Contents
Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,
Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
Experimental Design
Parts Needed
Parts from Registry
Promoters
pR R0051
pLacIQ1 K091112
pTetR R0040
pLuxR R0062
pMnt R0073
Genes
RBS+lamba cI+TT K082022
pBAD+RBS+LacI+TT S03980
pBAD+RBS+tetR+TT K091145
pBAD+RBS+luxR+TT K091179
RBS(B0030)+GFP E5500
RBS(B0030)+RFP S04120
Mnt C0072
LuxO-P K131022
Parts needed to be built from scratch
Pprp sequence within the prp operon[1]
prpB 2-methylisocitrate lyase in E.coli MG1655 [2]
pLuxT
frame shift suppressor 1
frame shift suppressor 2
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [3]
luxO luxT [4]
PprpB propionate [5]
