Davidson Projects with Updates
Contents
Building LuxI sender TESTER cell
R0011 (verified) + F1610 (not right part from registry)
Going after S03608 (pLac_RBS_LuxI) from 2008 paper registry :-(
If we get this and verify, we are done with this. If not, we are in need of LuxI part.
Building LuxR receiver TESTER cell
Part K09100 has been built.
Ready to test.
Building LasI sender TESTER cell
Building LasR receiver TESTER cell
Ligatiion 1A S03156 + R0011
Ligation 1B R0079 + E0240
Ligation 1C B0015 + R0079
Ligation 2: Successful Ligation 1A + uccessful Ligation 1B
Ligation 3: Successful Ligation 2 + R0011
Testing Crosstalk between Lux and Las
1) Mix LuxI sender cells with LasR receiver tester cells: negative control
2) Mix LasI sender cells with LuxR receiver tester cells: negative control
3) Mix LuxI sender cells with LuxR receiver tester cells: experiment we hope will glow green
4) Mix LasI sender cells with LasR receiver tester cells: experiment we hope will glow green
5) Verify LuxR receiver tester cells don't glow solo
5) Verify LasR receiver tester cells don't glow solo
