Suppressor Logic Toolkit and Quasi-Hash Function
Contents
Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,
Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
Experimental Design
Parts Needed
Parts from Registry
Promoters
pR R0051
pLacIQ1 K091112
pTetR R0040
pLuxR R0062
pMnt R0073
Genes
RBS+lamba cI+TT K082022
pBAD+RBS+LacI+TT S03980
pBAD+RBS+tetR+TT K091145
pBAD+RBS+luxR+TT K091179
RBS(B0030)+GFP E5500
RBS(B0030)+RFP S04120
Mnt C0072
Parts needed to be built from scratch
Pprp
prpB
pLuxT
frame shift suppressor 1
frame shift suppressor 2
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [1]
luxO luxT [2]
PprpB propionate [3]
