Suppressor Logic Toolkit and Quasi-Hash Function

Suppressor Logic Toolkit and Quasi-Hash Function

The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function

Basic Toolkit Design

The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,

Toolkit with Specific Inputs and Outputs

PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.

See references at bottom of page.

Experimental Design

Parts Needed

Parts from Registry

Promoters

pR R0051

pLacIQ1 K091112

pTetR R0040

pLuxR R0062

pMnt R0073

Genes

RBS+lamba cI+TT K082022

pBAD+RBS+LacI+TT S03980

pBAD+RBS+tetR+TT K091145

pBAD+RBS+luxR+TT K091179

RBS(B0030)+GFP E5500

RBS(B0030)+RFP S04120

Mnt C0072

LuxO-P K131022

Parts needed to be built from scratch

Pprp sequence within the prp operon[1]

prpB 2-methylisocitrate lyase in E.coli MG1655 [2]

pLuxT

frame shift suppressor 1

frame shift suppressor 2

References

Powerpoint File:Suppressorlogic3.ppt

Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [3]

luxO luxT [4]

PprpB propionate [5]