Ethanol Precipitation of Vector DNA

1. After digestion with restriction enzymes (ex. Normally a 40 µL digestion)
2. Add 1/10 the digestion volume of 3M NaOAc pH 5.2 (ex. 4 µL of NaOAc)
3. Add 2X the reaction volume of ethanol (ex. 88 µL of ethanol)
4. Vortex and put in -80°C freezer for 15 minutes
5. Centrifuge for 15 minutes (Place the tube hinge out in the microcentrifuge to know where the pellet is.
6. Dump off the liquid and allow to dry, either sitting on the bench or with the lyophilizer.