Status Of Building Parts

MWSU + Davidson Status of Building Sender and Receiver

plux-lac hybrid promoter:

• I751502
• In the process of building another plux-lac hybrid promoter that is repressed by HSL and lacI

Responsible: Robert Cool, Erin Feeney and Pallavi Penumetcha
Parts we have Mini-Preps and Digestions for

Part	Description	Size of Insert (w/ BioBrick ends)	Verification using PCR
BBa_F2620	Accepts 3OC6HSL as an input and outputs PoPS	1102 bp	Appears to be reversed with J04430
BBa_J04430	GFP generator switched on by IPTG	1124 bp	Appears to be reversed with F2620
BBa_P0440	Produces tetR	881 bp	Verified

Look at USTC 2007 for logic gates

T9002 - no colonies

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F2622 + E0240 (ligated/colonies coming up)

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S03632 (ligations needed)

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But BBa_S03632 is not available, so we will need to build our own version using the 3 parts below:
Parts: R0011 + C0261 + B0015

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R0011 + F1610 (minis, frag needed/colonies coming up, minis thurs)

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F2620 + E0240 (minis growing/ frag available)

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P0440

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