Suppressor Logic Toolkit and Quasi-Hash Function
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Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,
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Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
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Experimental Design
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Parts Needed
Promoters
pR R0051
pLacIQ1 K091112
pTetR R0040
pLuxR R0062
Genes
lamba cI C0051
LacI C0012
RBS+tetR I15040
luxR C0062
GFP E0040
RFP E1010
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [1]
luxO luxT [2]
PprpB propionate [3]