Suppressor Logic Toolkit and Quasi-Hash Function

Suppressor Logic Toolkit and Quasi-Hash Function

The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function

Basic Toolkit Design

The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,

Toolkit with Specific Inputs and Outputs

PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.

See references at bottom of page.

Experimental Design

Parts Needed

Promoters

pR R0051

pLacIQ1 K091112

pTetR R0040

pLuxR R0062

Genes

lamba cI C0051

LacI C0012

RBS+tetR I15040

luxR C0062

GFP E0040

RFP E1010

References

Powerpoint File:Suppressorlogic3.ppt

Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [1]

luxO luxT [2]

PprpB propionate [3]