Suppressor Logic Toolkit and Quasi-Hash Function
Contents
Suppressor Logic Toolkit and Quasi-Hash Function
The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function
Basic Toolkit Design
The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors.
Toolkit with Specific Inputs and Outputs
PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.
See references at bottom of page.
Experimental Design
Parts Needed
Parts from Registry
Promoters
pR R00512009 Kit Plate 1 6K pSB1A2
pLacIQ1 K091112MWSU
pTetR R00402009 Kit Plate 1 6I pSB1A2
pLuxR R00622009 Kit Plate 1 6O pSB1A2
pMnt R00732009 Kit Plate 1 16B pSB2K3
Genes
RBS+lamba cI+TT P04512009 Kit Plate 2 1N pSB1AK3
pBAD+RBS+LacI+TT S03980MWSU
pBAD+RBS+tetR+TT K091145MWSU
pBAD+RBS+luxR+TT K091179MWSU
RBS(B0030)+GFP E55002007 Parts Kit Plate 1 9J pSB1A2
RBS(B0034)+RFP MWSU
Mnt C00722009 Kit Plate 1 12L pSB2K3
LuxO-P K1310222009 Kit Plate 3 8K pSB1AC3
Parts needed to be built from scratch
prpBp Ecocyc
(text version)
tgaaattaaa catttaattt tattaaggca attgtggcac accccttgct ttgtctttat caacgcaaat aacaagttga taaca
http://gcat.davidson.edu/GcatWiki/images/2/2f/PrpBp.JPG
prpB 2-methylisocitrate lyase in E.coli MG1655 [1]http://gcat.davidson.edu/GcatWiki/images/6/6c/PrpB.JPG
pLuxT [2]
http://gcat.davidson.edu/GcatWiki/images/e/e3/LuxT_upstream.JPG
frame shift suppressor 1 CUGUCCUAA
frame shift suppressor 2 UUACUAGAC
prpBp and prpB have no EcoR1, Xba1, Spe1, or Pst1 sites. Source www.neb.com
References
Powerpoint File:Suppressorlogic3.ppt
Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [3]
luxO luxT [4]
PprpB propionate [5]
