Blasting Spacers for Our Genome

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Blasting Spacers

CRISPR one results from CRISPR finder are shown below. I blasted all of the spacers using the nr/nt database and the results are shown below.

CrisperOne.jpg


Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG

SpacerOne.jpg

Matches by chance!

Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA

SpacerTwo.jpg

Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA

SpacerThree.jpg

Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG

SpacerFour.jpg SpacerFourTwo.jpg

Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC

SpacerFive.jpg

This image is only part of it. . .

SpacerFiveTwo.jpg


NOTE: lots of hits from each organism- indicates that multiple parts have been incorporated into our species' spacers. . . interesting to study what genes have been inserted

Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG

SpacerSix.jpg

No viral insertions in this spacer

Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG

SpacerSeven.jpg

Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC

SpacerEight.jpg

E-values not good- no significant hits!

Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC

SpacerNine.jpg

Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA

No significant hits. . . lowest E-value is 1.5

Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC

SpacerEleven.jpg

Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC

No significant hits. . . lowest E-value is 1.6

Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA

Spacer13.jpg

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