Ethanol Precipitation of Vector DNA

• After digestion with restriction enzymes (ex. Normally a 40 µL digestion) • 1/10 the digestion volume of NaOAc (ex. 4 µL of NaOAc) • 2X the reaction volume of ethanol (ex. 88 µL of ethanol) • Vortex and put in -80°C freezer for 15 minutes • Centrifuge for 15 minutes (Place the tube hinge out in the microcentrifuge to know where the pellet is. • Dump off the liquid and allow to dry, either sitting on the bench or with the lyophilizer.