DNA Sequencing at Iowa State University

• Prepare minipreps of DNA to be sequenced – concentration should be at least 50 ng/ul
• Put 10 ul of each DNA template in a 1.5 ml tube
• Label the DNA template tubes with a name not more than 8 characters
• Seal tubes with parafilm and place in 50 ml tube
• Wrap in bubblewrap
• Go to http://www.dna.iastate.edu/users/index.html
• Login = eckdahl, Password = toad
• Input number of reactions, choose Plasmid Template Type, hit Continue
• Input names of template exactly as written on tubes
• Input concentration in ug/ul (eg. 50 ng/ul = .050 ug/ul)
• Input approximate insert size and approximate vector size
• Input G00100 for Forward primer or G00101 for Reverse primer
• Input “Eckdahl primer box” under Comments
• Press Continue
• Address Error Message if you can
• Press Submit
• Print page with Samples Submitted and Tracking Number
• Place page and 50 tube(s) with DNA templates in FedEx box for shipping
• Get data from http://dna.biotech.iastate.edu/users/dna_gid.html