JP Feb 04 16
Big Picture Questions:
1. What do we want out of our research ; what would be a perfect outcome? How would we get there?
What's triggered in the first step of the postprandial organ increase cascade in the small intestine. A gene or combination of genes which is/are differentially regulated after feeding. Comparing pre- and postprandial gene normalized expressions. Narrowing the differentially expressed genes by process. Narrow by what's present in other research at what time points. We don't know if the gene we want continues expression the whole time of digestion (regulation) or just triggers an initial response. We don't know if the process turns a gene off to allow the response, or turns a gene on.
2. What are we going to do with each of our 12 data sets to evaluate & treat it later?
We have normalized data. We need to analyze differential expression. We need to confirm that we can find intestine specific genes in all 6 samples. Serosa v Mucosa specific?
