Q5 PCR NEB

Reaction Setup

REAGENT	VOLUME (µL)	FINAL CONC.
10 µM Forward Primer	2.5 µL	0.5 µM
10 µM Reverse Primer	2.5 µL	0.5 µM
Template DNA	Y (1ng)	1 ng
Q5 High-Fidelity 2X Master Mix	25 µL	1X
Nuclease-free Water	20- Y

Final Volume = 50µL

Notes:

• Q5 High-Fidelity 2X Master Mix has an error rate > 100-fold lower than that of Taq DNA Polymerase

• Q5 High-Fidelity 2X Master Mix can be stored at -20°C. If precipitate forms after thawing, resuspend before use

• Remember to first dilute primers in nuclease-free water if starting with a higher concentration

• Do not exceed 50 µL final volume