Davidson Missouri W/Davidson Protocols

1. http://www.bio.davidson.edu/courses/molbio/labnotebook.html How to Keep a Lab Notebook]
2. Common molecular reagents
3. Standard Assembly
4. Compatibility of Plasmids
5. Building dsDNA with Oligos
6. Setting up PCR mixtures
7. PCR and Mg²⁺ concentration
8. Clean and Concentrate DNA (after PCR, before digestion)
9. Pouring an agarose gel
10. Calculate MWs
11. Digest DNA with restriction enzymes
12. Double Digest Guide
13. Ethanol Precipitate DNA (short protocol)
14. 1kb MW markers
15. Shrimp Alkaline Phosphatase
16. Qiagen QIAquick Gel Purification
17. Qiagen QIAquick Column Regeneration Protocol
18. ElectroElute Gel Purification
19. Ligation Protocol
20. Heat Shock Transformation OR Short version of Heat Shock
21. Zippy Transformation
22. Colony PCR to Screen for Successful Ligations
23. Promega miniprep
24. Choices for Transformation: Heat Shock vs. Zyppy
25. Choices for Mini-Preps: Promega vs. Zyppy
26. Sequencing at CUGI
27. Making dsDNA Using Primer Dimers