Wet Lab Pages

This is the space for MWSU and DC wet lab students to create content. Our part numbers will be in this range only: BBa_K091000 to BBa_K091999.

1. Davidson Wet Lab Protocols
2. MWSU Wet Lab Protocols
3. Build a LuxR Repressor PDF Reprint
4. Plasmid Compatability
5. Davidson -80 Stocks
6. Using Amp and Amp^R as communication
7. FAQs for Wikis

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Registry parts to clone

Things to Do

1. Experiment with making lines of media in petri dishes.
2. Determine concentration of HSL needed to turn on our receiver + reporter. Compare with BBa_T9002
3. Determine what percent of a colony need to be sender cells in order for an entire colony to become switched on by HSL.
4. We need to make sender and receiver cells (using existing sub-parts such as F1610), and/or take from registry (e.g., BBa_T9002)
5. Make a derived pLac promoter that will permit LuxR+HSL to be a repressor to be used in combination with Lux pR promoter. See this paper.
6. Make an Amp^R sender device.
7. Design experiments to test the distance HSL and beta lactamase can diffuse in agar and over what time spans.

Composite Parts:

Part	Description	Student Responsible (DC or MW)	Location and Vector	Resistance	Results from 5/22/08
BBa_F2621	AHL receiver with lux pR and codes for LuxR	Karlesha Roland (DC)	1001 12B pSB1A2	amp	growth MW
BBa_I15030	Lux-sender(Autoinducing)Codes for LuxI and LuxR	Kelly Davis (DC) will this send to part BBa_I13263	1015 8F pSB3K3	kan	growth MW
BBa_I13263	Lux Receiver (HSL & R0063 driven)produces YFP	Pallavi Penumetcha (DC)	1002 8E pSB1A2	amp	growth MW & DC
BBa_f2622	3OC6HSL receiver that outputs PoPS	James Barron (DC)	1001 12E pSB1A2	amp	no growth
BBa_F1610	Device that receives PoPS and outputs LuxI	Malcolm Campbell (DC)	1016 10D pSB1AK3	amp & kan	growth MW
BBa_I0426	Las-reciver(EYFP)	Kristi Muscalino (DC)	1008 5B pSB1A2	amp	growth MW & DC
BBa_I0407	LasI test	Erin Feeney (DC)	1008 4F pSB1A2	amp	growth MW & DC
BBa_I0466	RhlR Protein Generator without LVA	Laurie Heyer (DC)	1001 5C pSB1A2	amp	growth MW

Basic Parts:

Part	Description	Student Responsible (DC or MW)	Location and Vector	Resistance	Results from 5/22/08
BBa_C0061	LuxI gene with LVA	Madeline Parra (DC)	1000 3E pSB1A2	amp	growth MW
BBa_C0161	LuxI gene without LVA	Xiao Zhu (MW)	1001 8B pSB1A2	amp
BBa_C0062	LuxR gene without LVA	John Igo (MW)	1000 3F pSB1A2	amp
BBa_R0063	lux pL	Aaron Lewis (MW)	1000 4H pSB1A2	amp
BBa_R0062	lux pR	Andrew Gordon(MW)	1000 4G pSB1A2	amp
BBa_C0070	rhlI gene	Jeff Poet (MW)	1013 3E pSB2K3	kan
BBa_C0071	rhlR gene with LVA	Max Win (DC)	1013 3F pSB2K3	kan	no growth
BBa_C0171	rhlR gene without LVA	MWSU	1001 6F pSB1A2	amp
BBa_R0079	las pR	Todd Eckdahl (MW)	1001 10F pSB1A2	amp
BBa_C0079	lasR gene	Robert Cool (MW)	1013 4F pSB2K3	kan
BBa_C0078	LasI gene	Alicia Allen (MW)	1013 4E pSB2K3	kan
BBa_J07019	fecA promoter with Fur box	Not in Registry
BBa_####	Description	Sven(MW)
BBa_####	Description	Sven(MW)
BBa_####	Description	Sven(MW)

Fluorescent Protein Gradient Testers:

Part	Description	Student Responsible (DC or MW)	Location and Vector	Resistance	Results
BBa_J04450	Lac-RBS-RFP-T	Robert Cool (MW)	1004 4f pSB1A2	amp
BBa_J04451	pLac-RBS-RFP w/ LVA tag-T-T	Erin Feeney and Kelly Davis (DC)	1016 9F pSB1AK3	amp and kan
BBa_I13520	pBAD-RBS-mRFP-T-T	Erin Feeney and Kelly Davis (DC)	1009 7B pSB1A2	amp
BBa_J5528	pBAD-RBS-GFP-T-T	Erin Feeney and Kelly Davis (DC)	1015 7A pSB2K3	kan
BBa_J04430	pLac-RBS-GFP-T-T	Erin Feeney and Kelly Davis (DC)	1004 4H pSB1A2	amp

Parts for Sending and Receiving Signals:

Part	Description	Student Responsible (DC or MW)	Location and Vector	Resistance	Results
BBa_J07019	fec A promoter with fur box	James Barron (DC)	Not in Registry-Needs to be amplified
BBa_F2622	3OC6HSL Receiver Device	Pallavi Penumetcha (DC)	1001 12e pSB1A2	amp
BBa_I14032	pLac promoter	Pallavi Penumetcha (DC)	1013 8e pSB2K3	kan
BBa_S03632	lacI promoter and luxI gene	Pallavi Penumetcha (DC)	1016 7f pSB1AK3	amp and kan
BBa_E0240	RBS and GFP	Pallavi Penumetcha (DC)	1001 4B pSB1A2	amp