LuxR/cI
From GcatWiki
Parallel Biobrick Assembly
Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)
K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]
| Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
| 1A | B0015 | Double Terminator | RX/SP | RX | 129bp | C0072 | Mnt Gene | RX/SP | RS | 288bp | ||
| 1B | B0034 | RBS | RX/SP | RX | 12bp | K091109 | LuxS Gene | RX/SP | RS | 516bp | ||
| 2 | B0034 | RBS | RX/SP | SP | 12bp | Product of 1B | LuxS + RBS | RX/SP | XP | |||
| 3 | Product of 2 | RBS + LuxS + RBS | RX/SP | SP | Product of 1A | Mnt Gene + TT | RX/SP | XP | ||||
| 4 | K091107 | pLux/cI | RX/SP | SP | Product of 3 | RBS + LuxS + RBS + Mnt + TT | RX/SP | XP | Objective 1 |
Parts
| BBa_ | Description | Source | location('07) | Vector |
|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 |
| B0034 | RBS | registry 07 | Plt.1-3O | pSB1A2 |
| C0072 | Mnt Gene | registry 07 | Plt.2-5M | pSB2K3 |
| K091107 | pLux/cI | N/A | ||
| K091109 | LuxS | N/A |
| Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
| 1 | ||||||||||||
