Difference between revisions of "APe Sequence Analysis"

Revision as of 15:39, 19 July 2017

Download the free ApE software if you want it on your own computer. All college-owned computers already have ApE installed so you can use those computers if you prefer. This tool is used by researchers all over the world.
Access your sequence files. Click once on a file with the .seq suffix. For Macintosh computers, hold down the command button and type i. A window will open. About halfway down the window under the heading "Open With", choose ApE as the application. Then click on the "Change All" button so that all .seq files will automatically open with ApE.
Repeat step 2 above but use a .ab1 file instead of .seq file.
Download the 4 receiving plasmids and locate which one you used. Open that .ape file.
Open the appropriate .seq file for your eXperimental samples.

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 # Access your sequence files. Click once on a file with the .seq suffix. For Macintosh computers, hold down the command button and type i. A window will open. About halfway down the window under the heading "Open With", choose ApE as the application. Then click on the "Change All" button so that all .seq files will automatically open with ApE.
 # Repeat step 2 above but use a .ab1 file instead of .seq file.
-#Open the appropriate .seq file for your eXperimental samples. Also open the related receiving plasmid ApE file (pClone Red, rClone Red, actClone Red, or repClone Red).
+# Download the [http://www.bio.davidson.edu/people/macampbell/113/weekly_Labs/plasmids.zip 4 receiving plasmids] and locate which one you used. Open that .ape file.
+# Open the appropriate .seq file for your eXperimental samples.