Difference between revisions of "Applications of Ribozymes in Synthetic Systems - Danielle Jordan"
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== [[History and Background]] == | == [[History and Background]] == | ||
== How do you make artificial ribozymes? == | == How do you make artificial ribozymes? == | ||
| − | The method of directed evolution is used to create specific ribozymes. Large quantities of RNA are produced using polymerase enzymes. The large library of ribozymes are mutated and amplified using error prone rtPCR (reverse transcriptase PCR). One method of selection is by using biotin tags, which are covalently bonded to a particular substrate and can then be extracted by streptavidin-magnetic beads. | + | The method of directed evolution is used to create specific ribozymes. Large quantities of RNA are produced using polymerase enzymes. The large library of ribozymes are mutated and amplified using error prone rtPCR (reverse transcriptase PCR). One method of selection is by using biotin tags, which are covalently bonded to a particular substrate and can then be extracted by streptavidin-magnetic beads. Thus, the molecules that exhibit the optimal ligase activity are recoved using the streptavidin matrix. |
Revision as of 03:26, 20 November 2007
What are ribozymes?
Ribozymes, also known as RNA enzymes or catalytic RNA, are RNA molecules that cataylze chemical reactions. They are able to catalzye hydrolysis of their own phosphodiester bonds or other RNA bonds. Some, such as RNA polymerase ribozymes, are able to catalyze their own synthesis.
History and Background
How do you make artificial ribozymes?
The method of directed evolution is used to create specific ribozymes. Large quantities of RNA are produced using polymerase enzymes. The large library of ribozymes are mutated and amplified using error prone rtPCR (reverse transcriptase PCR). One method of selection is by using biotin tags, which are covalently bonded to a particular substrate and can then be extracted by streptavidin-magnetic beads. Thus, the molecules that exhibit the optimal ligase activity are recoved using the streptavidin matrix.
