BioBrick Ligations

• For a 40 µL Digestion o 2000 ng of DNA in an appropriate volume o 4 µL of BSA (10x) o 4 µL of NEB 2 (10X Buffer) o 2 µL of EcoRI or XbaI o 2 µL of SpeI or PstI o A volume of dH2O to make a total volume of 40 µL