Difference between revisions of "Biology"
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Macampbell (talk | contribs) (Created page with "'''Topics to Investigate''' <u>High Priority = more people</u> * eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out. <br> Which plas...") |
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include drug resistance gene<br> | include drug resistance gene<br> | ||
test JGG design<br> | test JGG design<br> | ||
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* test fitness module with adhE | * test fitness module with adhE | ||
compare with tetA<br> | compare with tetA<br> | ||
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* swap out different components (promoters, RBS, alleles) | * swap out different components (promoters, RBS, alleles) | ||
* test out programmed evolution | * test out programmed evolution | ||
| + | * Stress module needs more work (see eCDM8 outputs)<br> | ||
Revision as of 14:26, 30 May 2013
Topics to Investigate
High Priority = more people
- eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out.
Which plasmid carries which device?
- Determine the junction sequences and associated primers
(use existing rules)
build scaffold for insert segments
include orI (collaborate with copy number research)
include drug resistance gene
test JGG design
- test fitness module with adhE
compare with tetA
fine tune tetA resistance
combine with tetA
- Copy number of plasmids
Can we alter Ori to produce a range of plasmid densities in E. coli?
- Does theophylline diffuse across the membrane and thus the system fails
Second Order Priority = one person
- produce different CDM alleles
- swap out different components (promoters, RBS, alleles)
- test out programmed evolution
- Stress module needs more work (see eCDM8 outputs)
