Blasting Spacers for Our Genome

From GcatWiki

Revision as of 17:29, 5 November 2009 Karicheson (Talk | contribs) Blasting Spacers ← Previous diff		Revision as of 17:32, 5 November 2009 Karicheson (Talk | contribs) Blasting Spacers Next diff →
Line 58:		Line 58:
	Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA		Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA
-	[[Image:spacerTen.jpg]]	+	No significant hits. . . lowest E-value is 1.5
		+
		+	Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC
		+
		+	[[Image:spacerEleven.jpg]]
		+
		+	Spacer Twelve:

---

Revision as of 17:32, 5 November 2009

Blasting Spacers

CRISPR one results from CRISPR finder are shown below. I blasted all of the spacers using the nr/nt database and the results are shown below.

Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG

Matches by chance!

Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA

Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA

Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG

Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC

This image is only part of it. . .

NOTE: lots of hits from each organism- indicates that multiple parts have been incorporated into our species' spacers. . . interesting to study what genes have been inserted

Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG

No viral insertions in this spacer

Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG

Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC

E-values not good- no significant hits!

Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC

Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA

No significant hits. . . lowest E-value is 1.5

Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC

Spacer Twelve: