Blasting Spacers for Our Genome

From GcatWiki

Revision as of 17:39, 5 November 2009 Karicheson (Talk | contribs) Blasting Spacers ← Previous diff		Revision as of 17:41, 5 November 2009 Karicheson (Talk | contribs) Blasting Spacers Next diff →
Line 71:		Line 71:
	[[Image:spacer13.jpg]]		[[Image:spacer13.jpg]]
		+
		+	Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC
		+
		+	[[Image:spacer14.jpg]]

---

Revision as of 17:41, 5 November 2009

Blasting Spacers

CRISPR one results from CRISPR finder are shown below. I blasted all of the spacers using the nr/nt database and the results are shown below.

Spacer One: TGCGTCGTCCGGTGGCCGTCAATAAATGTCGCAAGGG

Matches by chance!

Spacer Two: TCCTACGACCTCGTCGGCGTCAACGGCTGGCCCGA

Spacer Three: CACCCTACAACAGGTGAAATCTACCAGACAAAAGA

Spacer Four: TCACCCAAGCGCAAGCAACAGCTGATCGAGGACCTG

Spacer Five: GCGACGGCGGCCAGTTCCGCGAGGGCGGGAAGGTCC

This image is only part of it. . .

NOTE: lots of hits from each organism- indicates that multiple parts have been incorporated into our species' spacers. . . interesting to study what genes have been inserted

Spacer Six: TGCGAGTGTTGCGGGGAACCGACTCGGGTAGGCCAG

No viral insertions in this spacer

Spacer Seven: ACGGTTTCGCTACCACCATCGCCACCAGCAACTGCCG

Spacer Eight: GACGAGTATGCCAACCGGCTCGTGAGCGGGCGC

E-values not good- no significant hits!

Spacer Nine: TGGTAGGCGTCGTAGGTGTTCGTGGCGAGCGTGTC

Spacer Ten: ACTCACTGGATTATGACCCCTACAACGAGGGCGTCA

No significant hits. . . lowest E-value is 1.5

Spacer Eleven: GGATCTCGATCGTTGTAGTATCCATAGCTGCTATACC

Spacer Twelve: GAAGTAACGCAACTCCAGTGAGCGCTACTGAGAGCCC

No significant hits. . . lowest E-value is 1.6

Spacer Thirteen:CCGATCACGCCCTGCCGATACTGGTAGTTCGCGATA

Spacer Fourteen: TCGTCGGCCGGCTCGTCGGCCGACGTGGACTTGC