Difference between revisions of "Chalkboard of Ideas"
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* Random number generator (perhaps binary - 0,1 = two reporters) | * Random number generator (perhaps binary - 0,1 = two reporters) | ||
* Noise threshold - if noise level above measure wanted, amplify it causing death to all those cells --> tight controlled system | * Noise threshold - if noise level above measure wanted, amplify it causing death to all those cells --> tight controlled system | ||
| − | + | # Maybe tie system to Tet resistance or something similar? - die if too low or too high of output (but tunable by protein threshold, not user) | |
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== Mechanisms to Use to Create Noise == | == Mechanisms to Use to Create Noise == | ||
Revision as of 18:27, 22 February 2010
Contents
Characteristics to Utilize with Noise
- Differentiation (bimodal populations)
- Oscillatory circuit
- Quorum sensing
- Aging / Death (selective advantage of noisy system?)
Possible Applications of Noise
- Battleship - randomly survey area until get a hit, spread out from that point
- Combination lock - random generation of lock, random opening of lock - survey possibilities until something clicks
- Maze
- Biosensor
- Random number generator (perhaps binary - 0,1 = two reporters)
- Noise threshold - if noise level above measure wanted, amplify it causing death to all those cells --> tight controlled system
- Maybe tie system to Tet resistance or something similar? - die if too low or too high of output (but tunable by protein threshold, not user)
Mechanisms to Use to Create Noise
- Positive feedback loops
- Long cascades
- Transcriptional bursting? (promoter manipulation)
- Translational bursting? (codon usage, ribosome binding affinity?)
- Carbonylation of proteins - causes rapid degradation - could be useful in stochasticity in degradation rate
Usable Parts
- Freed collection of high-noise promoters
- ppGpp (Guanosine tetraphosphate) - an alarmone nucleotide produced by two synthetases that controls the activity of sigma transcription factors in survival and senescence/cross-protection pathways (competitive sigma factor binding) Con = ppGpp is necessary in many amino acid synthesis pathways, which might be a problem if manipulating ppGpp levels - or might not be
- RecA / LexA - protease / repressor (could be used as a way to randomly quiet and turn on genes. Con = RecA involved in stress response - impt in wt cells, could be detrimental to manipulate
- Lux operon - quorum sensing - synchronized behaviour
