Chalkboard of Ideas
From GcatWiki
Contents
Characteristics to Utilize with Noise
- Differentiation (bimodal populations)
- Oscillatory circuit
- Quorum sensing
- Aging / Death (selective advantage of noisy system?)
Possible Applications of Noise
- Battleship - randomly survey area until get a hit, spread out from that point
- Combination lock - random generation of lock, random opening of lock - survey possibilities until something clicks
- Maze
- Biosensor
- Random number generator (perhaps binary - 0,1 = two reporters)
- Noise threshold - if noise level above measure wanted, amplify it causing death to all those cells --> tight controlled system
Mechanisms to Use to Create Noise
- Positive feedback loops
- Long cascades
- Transcriptional bursting? (promoter manipulation)
- Translational bursting? (codon usage, ribosome binding affinity?)
Usable Parts
- RecA / LexA - protease / repressor (could be used as a way to randomly quiet and turn on genes. Con = RecA involved in stress response - impt in wt cells, could be detrimental to manipulate
- Lux operon - quorum sensing - synchronized behaviour
