Construction of a genetic toggle switch in E. coli

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Authors

Timothy Gardner
Charles Cantor
James Collins

Methods

Flow Cytometry

Supplementary Material

Supplementary Material

Helpful Information

If you are having trouble determining the meaning of Type I-IV plasmids, look at the first page of the supporting materials [1]. Each plasmid type has a different construction and purpose. Types I-III serve as control plasmids, while Type IV plasmids are the ones responsible for the toggle switch.