DNA Sequencing at Eurofins

1. Prepare template and primer solutions at the concentrations listed at the bottom of this page.
2. Grab the appropriate number of bar-code tubes to be sent off for sequencing.
3. Record the code on each tube and what clone will be in it inside your lab notebook.
4. Get 8 microliters of your sample and add it to the correct bar-code tube. This can be done by putting the tip through the septum and ejecting the sample onto the inner wall of the tube.