Davidson Missouri W/Backwards promotion and read-through transcription

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We will also produce two constructs for tetsing promoters. MWSU will produce (Kan, RFP, Tet) while Davidson will produce (Kan, Tet, RFP). We can drop in different promoters and look for phenotypes.

Davidson is also going to have synthesized an improved pLac promoter that is shorter, will have better repression, better induction, and lack the backwards promotion we have detected with Part: BBa_R0010. We will test out the modified promoter BBa_R0011 which is reported to have good repression and strong induction. We may still introduce the UV5 double mutation to enhance transcription and compare with R0010.

MWSU is also going to produce backwards LacIq to put upstream of pLac BBa_R0010. The purpose of this is to have more LacIq in the cytoplasm at all times, regardless of ITPG status.

MWSU is also testing two of the one-time-flippable HixC sites produced by Bruce spring 2007.

MWSU is going to produce a new Hin-LVA expression plasmid that has ColE1 ori and uses Tet as the selectable marker.

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