Difference between revisions of "Davidson Missouri W/CUGI Sequencing"

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(For Dr. Campbell's Lab)
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The sequencing account's password at Clemson is the same as the name of the computer next to the microarray scanner. The website is [http://www.genome.clemson.edu www.genome.clemson.edu]
 
The sequencing account's password at Clemson is the same as the name of the computer next to the microarray scanner. The website is [http://www.genome.clemson.edu www.genome.clemson.edu]
  
*You need to send at least 30 ng of DNA in a tube for each sequencing reaction.
+
*You need to send at least 30 ng of DNA in a tube for each sequencing reaction. Unless you have a good reason not to, always submit 90 ng for each primer used for sequencing.  
 
*The reaction volumes are 5 µl, therefore, the DNA has to be dry or in a small volume.  
 
*The reaction volumes are 5 µl, therefore, the DNA has to be dry or in a small volume.  
*The DNA to be sequenced must be sent dried down, or within 3 µl.
+
*The DNA to be sequenced must be sent dried down, or within 3 µl. Unless you have a good reason not to, always submit the DNA dried down.  
*Primers are at 1 pmol/reaction
+
*Primers are at 1 pmol/reaction. Unless you have a good reason not to, always submit 3 pmols for each reaction you want performed.
 
*$3.00 / sample
 
*$3.00 / sample
  
 
Note by ATM:
 
Note by ATM:
 
I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions.
 
I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions.

Revision as of 17:28, 3 March 2009

For Dr. Campbell's Lab

The sequencing account's password at Clemson is the same as the name of the computer next to the microarray scanner. The website is www.genome.clemson.edu

  • You need to send at least 30 ng of DNA in a tube for each sequencing reaction. Unless you have a good reason not to, always submit 90 ng for each primer used for sequencing.
  • The reaction volumes are 5 µl, therefore, the DNA has to be dry or in a small volume.
  • The DNA to be sequenced must be sent dried down, or within 3 µl. Unless you have a good reason not to, always submit the DNA dried down.
  • Primers are at 1 pmol/reaction. Unless you have a good reason not to, always submit 3 pmols for each reaction you want performed.
  • $3.00 / sample

Note by ATM: I sent 80 ng of DNA for 3 samples and 10 pmol total of primer for 3 reactions.