Davidson Missouri W/Controlling Expression

To Solve the Hamiltonian Path Problem our team needs to produce mechanism that is capable to transcribing a sequence of adjacent genes. Due to the "flippable" nature of our model, promoter inserted after the start of transcription would not be practical becuase our team would loose control of the system and in turn the ability to obtain reproducible results. Because of our inability to control gene expression after the start of downstream of the start of transcription we searched for promoters of the highest processivity and repressibility. Thanks to the biobrick system we could choose from any operon in the E.Coli genome.

pLac
The promoter of the Lac operon was an optimal place to start becuase the kinetics of control are well documented in comparison to most E.Coli operons.