Difference between revisions of "Davidson Missouri W/Davidson Protocols"

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# http://www.bio.davidson.edu/courses/molbio/labnotebook.html How to Keep a Lab Notebook]
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# [http://www.bio.davidson.edu/courses/molbio/labnotebook.html How to Keep a Lab Notebook]
 
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents]
 
# [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents]
 
# [http://parts.mit.edu/registry/index.php/Assembly:Standard_assembly Standard Assembly]
 
# [http://parts.mit.edu/registry/index.php/Assembly:Standard_assembly Standard Assembly]

Revision as of 12:25, 20 May 2008

  1. How to Keep a Lab Notebook
  2. Common molecular reagents
  3. Standard Assembly
  4. Compatibility of Plasmids
  5. Building dsDNA with Oligos
  6. Setting up PCR mixtures
  7. PCR and Mg2+ concentration
  8. Clean and Concentrate DNA (after PCR, before digestion)
  9. Pouring an agarose gel
  10. Calculate MWs
  11. Digest DNA with restriction enzymes
  12. Double Digest Guide
  13. Ethanol Precipitate DNA (short protocol)
  14. 1kb MW markers
  15. Shrimp Alkaline Phosphatase
  16. Qiagen QIAquick Gel Purification
  17. Qiagen QIAquick Column Regeneration Protocol
  18. ElectroElute Gel Purification
  19. Ligation Protocol
  20. Heat Shock Transformation OR Short version of Heat Shock
  21. Zippy Transformation
  22. Colony PCR to Screen for Successful Ligations
  23. Promega miniprep
  24. Choices for Transformation: Heat Shock vs. Zyppy
  25. Choices for Mini-Preps: Promega vs. Zyppy
  26. Sequencing at CUGI
  27. Making dsDNA Using Primer Dimers