Difference between revisions of "Davidson Missouri W/Davidson Protocols"
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#[http://gcat.davidson.edu/iGEM08/gelwebsite/gelwebsite.html Optimize your Gel] | #[http://gcat.davidson.edu/iGEM08/gelwebsite/gelwebsite.html Optimize your Gel] | ||
#[http://gcat.davidson.edu/iGEM07/genesplitter.html Gene Splitting Web Site] | #[http://gcat.davidson.edu/iGEM07/genesplitter.html Gene Splitting Web Site] | ||
| − | #[http://gcat.davidson.edu/ | + | #[http://gcat.davidson.edu/iGEM08/bbprimer.html PCR Primers w/ BioBricks] |
# [http://www.promega.com/biomath/calc11.htm Promega T<sub>m</sub> Calculator] | # [http://www.promega.com/biomath/calc11.htm Promega T<sub>m</sub> Calculator] | ||
# [http://partsregistry.org/AHL List of auto-inducers and their Sigma catalog numbers] | # [http://partsregistry.org/AHL List of auto-inducers and their Sigma catalog numbers] | ||
#[[Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]] | #[[Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]] | ||
#[http://gcat.davidson.edu/IGEM06/oligo.html Lance-olator Oligos for dsDNA assembly] | #[http://gcat.davidson.edu/IGEM06/oligo.html Lance-olator Oligos for dsDNA assembly] | ||
Revision as of 15:52, 11 July 2008
- How to Keep a Lab Notebook
- Common molecular reagents
- Standard Assembly
- BioBrick Ends
- Compatibility of Plasmids
- Building dsDNA with Oligos
- Setting up PCR mixtures
- PCR and Mg2+ concentration
- Clean and Concentrate DNA (after PCR, before digestion)
- Pouring an agarose gel
- Calculate MWs
- Digest DNA with restriction enzymes
- Double Digest Guide
- Ethanol Precipitate DNA (short protocol)
- 1kb MW markers
- Shrimp Alkaline Phosphatase
- Qiagen QIAquick Gel Purification
- Qiagen QIAquick Column Regeneration Protocol
- ElectroElute Gel Purification
- Ligation Protocol
- Heat Shock Transformation OR Short version of Heat Shock
- Zippy Transformation
- Colony PCR to Screen for Successful Ligations
- Promega miniprep
- Choices for Transformation: Heat Shock vs. Zyppy
- Choices for Mini-Preps: Promega vs. Zyppy
- Making dsDNA Using Primer Dimers
Web Tools We Use
