Davidson Missouri W/Davidson Protocols

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  1. How to Keep a Lab Notebook
  2. Common molecular reagents
  3. Using degradation tags on proteins such as GFP
  4. Standard Assembly
  5. BioBrick Ends
  6. Compatibility of Plasmids
  7. Building dsDNA with Oligos
  8. Setting up PCR mixtures
  9. PCR and Mg2+ concentration
  10. Clean and Concentrate DNA (after PCR, before digestion)
  11. Pouring an agarose gel
  12. Calculate MWs
  13. Digest DNA with restriction enzymes
  14. Double Digest Guide
  15. Ethanol Precipitate DNA (short protocol)
  16. 1kb MW markers
  17. Shrimp Alkaline Phosphatase
  18. Qiagen QIAquick Gel Purification
  19. Qiagen QIAquick Column Regeneration Protocol
  20. ElectroElute Gel Purification
  21. Ligation Protocol
  22. Heat Shock Transformation OR Short version of Heat Shock
  23. Zippy Transformation
  24. TSS Competent Cell Transformation
  25. Colony PCR to Screen for Successful Ligations
  26. Promega miniprep
  27. Choices for Transformation: Heat Shock vs. Zyppy
  28. Choices for Mini-Preps: Promega vs. Zyppy
  29. Making dsDNA Using Primer Dimers
  30. Genomic Insertion Protocol
  31. glycerolstocks How to Make Glycerol Stocks of Bacteria
  32. When inducing with IPTG, use 3 µL of stock (0.2 g/mL = 20% w/v) to every 1 mL of LB or other liquid.
  33. When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid.

Computer Tools We Use

  1. All Sites In One Place
  2. Optimize your Gel
  3. Gene Splitting Web Site
  4. PCR Primers w/ BioBricks
  5. Promega Tm Calculator
  6. List of auto-inducers and their catalog numbers
  7. Sequencing at Agencourt Bioscience
  8. Sequencing at CUGI
  9. Analyzing Sequences with aPe
  10. Lance-olator Oligos for dsDNA assembly
  11. Access the GCAT-alog of Davidson and MWSU DNA Freezer Stocks
  12. Using Apes (A Plasmid Editor)