Davidson Missouri W/Davidson Protocols

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  1. How to Keep a Lab Notebook
  2. Common molecular reagents
  3. Standard Assembly
  4. BioBrick Ends
  5. Compatibility of Plasmids
  6. Building dsDNA with Oligos
  7. Setting up PCR mixtures
  8. PCR and Mg2+ concentration
  9. Clean and Concentrate DNA (after PCR, before digestion)
  10. Pouring an agarose gel
  11. Calculate MWs
  12. Digest DNA with restriction enzymes
  13. Double Digest Guide
  14. Ethanol Precipitate DNA (short protocol)
  15. 1kb MW markers
  16. Shrimp Alkaline Phosphatase
  17. Qiagen QIAquick Gel Purification
  18. Qiagen QIAquick Column Regeneration Protocol
  19. ElectroElute Gel Purification
  20. Ligation Protocol
  21. Heat Shock Transformation OR Short version of Heat Shock
  22. Zippy Transformation
  23. TSS Competent Cell Transformation
  24. Colony PCR to Screen for Successful Ligations
  25. Promega miniprep
  26. Choices for Transformation: Heat Shock vs. Zyppy
  27. Choices for Mini-Preps: Promega vs. Zyppy
  28. Making dsDNA Using Primer Dimers
  29. Genomic Insertion Protocol
  30. glycerolstocks How to Make Glycerol Stocks of Bacteria
  31. When inducing with IPTG, use 3 µL of stock (0.2 g/mL = 20% w/v) to every 1 mL of LB or other liquid.
  32. When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid.

Computer Tools We Use

  1. All Sites In One Place
  2. Optimize your Gel
  3. Gene Splitting Web Site
  4. PCR Primers w/ BioBricks
  5. Promega Tm Calculator
  6. List of auto-inducers and their catalog numbers
  7. Sequencing at Agencourt Bioscience
  8. Sequencing at CUGI
  9. Analyzing Sequences with aPe
  10. Lance-olator Oligos for dsDNA assembly
  11. Access the GCAT-alog of Davidson and MWSU DNA Freezer Stocks