Davidson Missouri W/Web tool

The gene-flipping mechanism that powers our computer requires the positioning of HixC sites within reporter genes' coding sequences. Through the use of PCR, a gene can be split into two pieces with BioBrick ends attached at the extremities. This allows for the ligation with a HixC site in the middle. Our team has developed a web-based tool to automate the generation of these PCR primers. It also predicts the resultant DNA sequences for the two intermediary parts, the final part, and the resultant polypeptide. By using this program we hope to make the gene-splitting process faster and to remove the potential for human error.

Tutorial

What follows here is an explanation of how to use the web tool and what it does.