Diagnostic RP Digestion for Checking Insert Size
From GcatWiki
Diagnostic RP Digestion for Checking Insert Size
This procedure is for checking the insert size of a BioBrick part. It uses digestion with EcoRI + PstI.
- Label a 1.5 ml tube for each of the digestions to be set up.
- Transfer about 300 ng into each tube and make up the difference with pure dH2O to 6 ul. Or just use 6 ul of a miniprep.
- Prepare a master mix as follows:
