Diagnostic RP Digestion for Checking Insert Size

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Diagnostic RP Digestion for Checking Insert Size

This procedure is for checking the insert size of a BioBrick part. It uses digestion with EcoRI + PstI.

  1. Label a 1.5 ml tube for each of the digestions to be set up.
  2. Transfer about 300 ng into each tube and make up the difference with pure dH2O to 6 ul. Or just use 6 ul of a miniprep.
  3. Prepare a master mix as follows:
  10 ng vector x (insert size/vector size) x 6 = ng insert, eg. 10ng x (700 bp/2100 bp) x 6 = 20 ng
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