Difference between revisions of "Divide and Conquer Biological Challenges"

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(* We need to get the Lsr/AI-2 system working.)
(* We need to get the Lsr/AI-2 system working.)
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=== * We need to get the Lux/AHL system working. ===
 
=== * We need to get the Lux/AHL system working. ===
 
=== * We need to get the Lsr/AI-2 system working. ===
 
=== * We need to get the Lsr/AI-2 system working. ===
Here are '''NO''' Lsr parts in the registry: <br>
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'''Our summary of the Lsr system:''' [[Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system]]<br>
[[Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system]]
+
* There are '''NO''' Lsr parts in the registry: <br>
We will need to make BB parts for:<br>
+
*We will need to make BB parts for:<br>
LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.  
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# LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.  
Lsr promoter
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# Lsr promoter
LuxS that produces DPD that somehow is converted to ''R''-THMF.
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# LuxS that produces DPD that somehow is converted to ''R''-THMF.
How do we make AI-2?
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# How do we make AI-2?
  
 
=== * We do NOT need to use additional chemical input signals (''e.g.'', aTc and IPTG). We will use only AHL and AI-2 added exogenously. ===
 
=== * We do NOT need to use additional chemical input signals (''e.g.'', aTc and IPTG). We will use only AHL and AI-2 added exogenously. ===

Revision as of 21:04, 28 May 2008

IGEM2008 design.jpg

* We need to get the Lux/AHL system working.

* We need to get the Lsr/AI-2 system working.

Our summary of the Lsr system: Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system

  • There are NO Lsr parts in the registry:
  • We will need to make BB parts for:
  1. LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
  2. Lsr promoter
  3. LuxS that produces DPD that somehow is converted to R-THMF.
  4. How do we make AI-2?

* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.

* We need to have an XOR logic gate produced.

Existing Logic Gates

  • NOT: (insert links to examples here)
  • AND: (insert links to examples here)

* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.

What if AmpR is secreted and cells are not AmpR? This would prevent cells down the chain from responding too soon.