Difference between revisions of "Divide and Conquer Biological Challenges"

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(* We need to get the Lux/AHL system working.)
(* We need to have an XOR logic gate produced.)
Line 28: Line 28:
 
# [http://partsregistry.org/Part:BBa_I732917 I732917]\<br>
 
# [http://partsregistry.org/Part:BBa_I732917 I732917]\<br>
 
*Inverters:<br>
 
*Inverters:<br>
 +
''Self-Contained Inverters''
 
# [http://partsregistry.org/Part:BBa_J23040 J23040 (AHL-dependent inverter)]<br>
 
# [http://partsregistry.org/Part:BBa_J23040 J23040 (AHL-dependent inverter)]<br>
 +
''PoPS Output Inverters''
 
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_Q04121 Q04121 (lac inverter)]<br>
 
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_Q04121 Q04121 (lac inverter)]<br>
 
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_Q04400 Q04400 (tet inverter)]<br>
 
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_Q04400 Q04400 (tet inverter)]<br>

Revision as of 21:24, 28 May 2008

IGEM2008 design.jpg

* We need to get the Lux/AHL system working.

* We need to get the Lsr/AI-2 system working.

Our summary of the Lsr system: Davidson/Missouri_Western_iGEM2008#Lsr_.28AI-2.29_cell_signaling_system

  • There are NO Lsr parts in the registry:
  • We will need to make BB parts for:
  1. LsrR and LsrK which are adjacent to each other in the E. coli genome. We could amplify them together with a total size of over 2600bp.
  2. Lsr promoter
  3. LuxS that produces DPD that somehow is converted to R-THMF.
  4. How do we get cells to make AI-2?

* We do NOT need to use additional chemical input signals (e.g., aTc and IPTG). We will use only AHL and AI-2 added exogenously.

* We need to have an XOR logic gate produced.

Existing Logic Gates

  • NOT:
  1. I732205 (NOT/ Dual-repressed NOR)
  • AND: (insert links to examples here)
  • NAND:
  1. I732914
  • NOR:
  1. I732916
  2. I732917\
  • Inverters:

Self-Contained Inverters

  1. J23040 (AHL-dependent inverter)

PoPS Output Inverters

  1. Q04121 (lac inverter)
  2. Q04400 (tet inverter)

* We need to figure out how to get cells to communicate in a sequence and not stop growing too soon.

  • What if AmpR is secreted and cells are not AmpR? This would prevent cells down the chain from responding too soon.