File:Gibson Assembly Size Control.pptx
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Revision as of 17:30, 11 June 2012 by Dcarr1 (talk | contribs) (Experiment proposal: At Missouri Western we have designed and order primers that will allow us to test Gibson Assembly in our own lab and to see if it will work for our purposes. Using J119022 (a promoter in psb1a2) and J119044 (a terminator-Cdog-RFP cons)
Gibson_Assembly_Size_Control.pptx (file size: 411 KB, MIME type: application/zip)
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Experiment proposal: At Missouri Western we have designed and order primers that will allow us to test Gibson Assembly in our own lab and to see if it will work for our purposes. Using J119022 (a promoter in psb1a2) and J119044 (a terminator-Cdog-RFP construct in psb1a2 BR) we have designed 10 primers that will allow us to try and put J119022 in place of the terminator in J119044. This experiment includes 4 variations on the size of J119022 by using extra sequence in front of the promoter that goes into the vector.
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| Date/Time | Dimensions | User | Comment | |
|---|---|---|---|---|
| current | 17:33, 11 June 2012 | (411 KB) | Dcarr1 (talk | contribs) | Experiment proposal: At Missouri Western we have designed and order primers that will allow us to test Gibson Assembly in our own lab and to see if it will work for our purposes. Using J119022 (a promoter in psb1a2) and J119044 (a terminator-Cdog-RFP cons |
| 17:30, 11 June 2012 | (411 KB) | Dcarr1 (talk | contribs) | Experiment proposal: At Missouri Western we have designed and order primers that will allow us to test Gibson Assembly in our own lab and to see if it will work for our purposes. Using J119022 (a promoter in psb1a2) and J119044 (a terminator-Cdog-RFP cons |
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