File:ThyA on Amp plates.jpg

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ThyA_on_Amp_plates.jpg(311 × 543 pixels, file size: 18 KB, MIME type: image/jpeg)

We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in comparison with the negative control plated on amp. The negative control is just the plasmid that carries the kanamycin resistance, so it would have needed kanamycin to grow. Negative control on top. Experimental on the bottom.

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current18:13, 3 June 2014Thumbnail for version as of 18:13, 3 June 2014311 × 543 (18 KB)Tetaye (talk | contribs)We took the transformed cells with the thyA fitness module and plated them on just Ampicillin plates. This was to mitigate having to select for both kanamycin and ampicillin resistance. Only transformed cells were expected to grow and they did in compa...
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