Difference between revisions of "General Questions"
MaCampbell (talk | contribs) |
MaCampbell (talk | contribs) |
||
| Line 23: | Line 23: | ||
**protein function is unclear | **protein function is unclear | ||
**if "putative" or "possible" or "predicted" occur in all the significant Tigrfam, COG, or PFAM names<br> | **if "putative" or "possible" or "predicted" occur in all the significant Tigrfam, COG, or PFAM names<br> | ||
| − | *'''Specificity unclear''': Choose if only more general information on the function of a protein family is available | + | *'''Specificity unclear''': Choose if only more general information on the function of a protein family is available e.g., ABC transporter (but can't predict substrate) e.g., Histidine kinase (but can't predict target response regulator) |
| − | e.g., ABC transporter (but can't predict substrate) | ||
| − | e.g., Histidine kinase (but can't predict target response regulator) | ||
High confidence in function and specificity: Choose if all significant observations agree with each other | High confidence in function and specificity: Choose if all significant observations agree with each other | ||
**Clear function | **Clear function | ||
**Clear specificity | **Clear specificity | ||
Revision as of 15:35, 11 September 2008
Alternative ORFs
Q: What if there's a possible Shine-Dalgarno sequence and start codon on the opposing strand in the middle of the gene you're working on? Do you assume that the computer has already marked it as a possible gene or do youhave to examine it as well?
A: No, let's stick to our pre-marked genes. There are many ATGs in a genome, and many possible SD sequences. But if the ORF does not code for anything real, it is a red herring.
Alternative ORFs'
Q: What if there's a possible Shine-Dalgarno sequence and start codon on the opposing strand in the middle of the gene you're working on? Do you assume that the computer has already marked it as a possible gene or do youhave to examine it as well?
A: No, let's stick to our pre-marked genes. There are many ATGs in a genome, and many possible SD sequences. But if the ORF does not code for anything real, it is a red herring.
Q: How do we rate our judgment calls? Can we indicate confidence levels?
A:Confidence levels (listed low to high)
- Hypothetical protein: choose if selected "hypothetical protein" under "gene product"
- Conserved hypothetical protein: choose if selected "conserved hypothetical protein" under "gene product"
- Not enough info to select "Family Membership" or "Function unclear"
- no domains identified
- no family membership)
- Family membership: Choose if conserved hypothetical protein identified via PFam or TIGRfam as belonging to a protein family but function and specificity are unclear.
- Function unclear: Choose if
- protein domain is identified
- protein function is unclear
- if "putative" or "possible" or "predicted" occur in all the significant Tigrfam, COG, or PFAM names
- Specificity unclear: Choose if only more general information on the function of a protein family is available e.g., ABC transporter (but can't predict substrate) e.g., Histidine kinase (but can't predict target response regulator)
High confidence in function and specificity: Choose if all significant observations agree with each other
- Clear function
- Clear specificity
