Difference between revisions of "General Statistical Calculation Tool"
From GcatWiki
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(→Estimate Genome Size) |
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===Estimate Genome Size=== | ===Estimate Genome Size=== | ||
*http://seqanswers.com/forums/showthread.php?t=11434 | *http://seqanswers.com/forums/showthread.php?t=11434 | ||
| + | *http://www.dolphing.com/?p=508 | ||
*[http://gsizepred.sourceforge.net/ GSP] - incorporate the Bayesian framework and EM algorithm for the genome size prediction | *[http://gsizepred.sourceforge.net/ GSP] - incorporate the Bayesian framework and EM algorithm for the genome size prediction | ||
**People skeptical of this method http://seqanswers.com/forums/showthread.php?t=10988 | **People skeptical of this method http://seqanswers.com/forums/showthread.php?t=10988 | ||
Revision as of 14:49, 3 June 2011
Contents
Features
Calculate Distribution of Read Lengths
Calculate How Many Substrings
- http://genometools.org/index.html - for both k-mer calculation tool and substring tool
- Shustring (SHortest Unique subSTRING) paper
- Shulen - Program for Computing the Null-Distribution of Shortest Unique Substring Lengths in DNA Sequences
Calculate N50
- http://code.google.com/p/biopieces/wiki/calc_N50 - part of biopieces
- Perl Script - and step by step idea
- Python code
Calculate GC Content
Calculate k-mer Distributions
- http://genometools.org/index.html - for both k-mer calculation tool and substring tool
- Jellyfish - more recent k-mer counting tool
Estimate Genome Size
- http://seqanswers.com/forums/showthread.php?t=11434
- http://www.dolphing.com/?p=508
- GSP - incorporate the Bayesian framework and EM algorithm for the genome size prediction
- People skeptical of this method http://seqanswers.com/forums/showthread.php?t=10988
- Other Method - http://www.cmb.usc.edu/papers/msw_papers/msw-149.pdf
Motivation
http://phagesdb.org/sort/ - it looks like genome size and gc% roughly correlates to the phage cluster
