Difference between revisions of "Helix-Slipping"

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http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700003.jpeg
 
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Figure 4. "Regulatory properties of the strand-displacement information transmission mechanism. The color scheme corresponds to that used in Fig. 1 with the following exceptions: switching strand, red; competing strand, green. (A) Gene expression ON ribozyme switch platform, L2bulge1. (B) Gene expression OFF ribozyme switch platform, L2bulgeOff1. (C and D) The theophylline-dependent gene-regulatory behavior of L2bulge1 (ON switch) (C), L2bulgeOff1 (OFF switch) (D), and L2Theo (nonswitch control). Gene-expression levels are reported in fold as defined in SI Text and were normalized to the expression levels in the absence of effector." (Win and Smolke, 2007). ''Image Permission Pending.''
  
 
== Links ==
 
== Links ==

Revision as of 21:35, 6 December 2007

Helix-slipping uses a sequence that forms an imperfectly paired double-stranded stem that is flexible in its nucleotide base-pairing through a "slip-structure" mechanism. It does not allow for rational design and results in disruption or restoration of the hammerhead ribozyme in response to changes in the aptamer.


http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700003.jpeg

Figure 4. "Regulatory properties of the strand-displacement information transmission mechanism. The color scheme corresponds to that used in Fig. 1 with the following exceptions: switching strand, red; competing strand, green. (A) Gene expression ON ribozyme switch platform, L2bulge1. (B) Gene expression OFF ribozyme switch platform, L2bulgeOff1. (C and D) The theophylline-dependent gene-regulatory behavior of L2bulge1 (ON switch) (C), L2bulgeOff1 (OFF switch) (D), and L2Theo (nonswitch control). Gene-expression levels are reported in fold as defined in SI Text and were normalized to the expression levels in the absence of effector." (Win and Smolke, 2007). Image Permission Pending.

Links

Ribozyme Switch

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