Difference between revisions of "Helix-Slipping"
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Helix-slipping uses a sequence that forms an imperfectly paired double-stranded stem that is flexible in its nucleotide base-pairing through a "slip-structure" mechanism. It does not allow for rational design and results in disruption or restoration of the hammerhead ribozyme in response to changes in the aptamer. | Helix-slipping uses a sequence that forms an imperfectly paired double-stranded stem that is flexible in its nucleotide base-pairing through a "slip-structure" mechanism. It does not allow for rational design and results in disruption or restoration of the hammerhead ribozyme in response to changes in the aptamer. | ||
| − | Helix-slipping only results in | + | Helix-slipping only results in OFF switches due to this lack of rational design. |
http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700003.jpeg | http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700003.jpeg | ||
Revision as of 21:49, 6 December 2007
Helix-slipping uses a sequence that forms an imperfectly paired double-stranded stem that is flexible in its nucleotide base-pairing through a "slip-structure" mechanism. It does not allow for rational design and results in disruption or restoration of the hammerhead ribozyme in response to changes in the aptamer. Helix-slipping only results in OFF switches due to this lack of rational design.
http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700003.jpeg
Figure 4. "Regulatory properties of the helix-slipping information transmission mechanism. The color scheme corresponds to that used in Fig. 1 with the following exception: communication module sequence, orange. (A) Gene expression OFF ribozyme switch platform based on helix slipping, L2cm4. The base stem of the aptamer was replaced with a communication module. (B) Regulatory activities of helix-slipping-based ribozyme switches. Gene-regulatory effects of the OFF switches at 5 mM theophylline are reported in fold repression relative to expression levels in the absence of effector. The corresponding communication module sequences are indicated. Gene expression levels are reported as described in Fig. 2." (Win and Smolke, 2007). Image Permission Pending.
