Difference between revisions of "Kathryn"
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(Created page with "THIS IS KATHRYN'S PAGE") |
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THIS IS KATHRYN'S PAGE | THIS IS KATHRYN'S PAGE | ||
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| + | '''Notes 1/12/16''' | ||
| + | Only .1 g of organ taken: possibility that connective tissue was taken, not representative of entire organ possibly | ||
| + | total RNA > mRNA using beads that attach to polyA tails of mRNA | ||
| + | - randomly fragment mRNA (since you can only read from an end to 75 base pairs) now get a lot more accurate reads, now know more about entire sequence | ||
| + | mRNA > CDNA: using reverse transcriptase, dNTP, use primers that has every possibly combination of 6 nucleotides so all mRNA is transcribed | ||
| + | CDNA: has been transcribed as mRNA and then changed into DNA to make more stable form | ||
Revision as of 19:52, 12 January 2016
THIS IS KATHRYN'S PAGE
Notes 1/12/16
Only .1 g of organ taken: possibility that connective tissue was taken, not representative of entire organ possibly
total RNA > mRNA using beads that attach to polyA tails of mRNA
- randomly fragment mRNA (since you can only read from an end to 75 base pairs) now get a lot more accurate reads, now know more about entire sequence
mRNA > CDNA: using reverse transcriptase, dNTP, use primers that has every possibly combination of 6 nucleotides so all mRNA is transcribed
CDNA: has been transcribed as mRNA and then changed into DNA to make more stable form
