Kathryn

THIS IS KATHRYN'S PAGE

Notes 1/12/16 Only .1 g of organ taken: possibility that connective tissue was taken, not representative of entire organ possibly total RNA > mRNA using beads that attach to polyA tails of mRNA - randomly fragment mRNA (since you can only read from an end to 75 base pairs) now get a lot more accurate reads, now know more about entire sequence mRNA > CDNA: using reverse transcriptase, dNTP, use primers that has every possibly combination of 6 nucleotides so all mRNA is transcribed CDNA: has been transcribed as mRNA and then changed into DNA to make more stable form