Ligation and Transformation

Ligation and Transformation

1. Plan to do a ligation with the vector only and one with vector + insert
2. Dilute vector to 10 ng/ul for use in the ligation
3. Calculate the amount of insert needed with the following formula

  10 ng vector x (insert size/vector size) x 6 = ng insert

Go to the Registry and choose "Add a part"

1. Decide whether the new part is a Basic Part (discrete function units that cannot be subdivided, like promoter, RBS, coding sequence), Composite Part (functional unit composed of basic parts, like gene expression cassette, inverter), or Construction Intermediate (no function, byproduct of construction).
2. Choose the number for the next available part
3. Be sure to include short and long descriptions of the part
4. Once data is available on the part, go back and include it as "Experience"