Difference between revisions of "Lipases"
| Line 1: | Line 1: | ||
Lipases in H. utahensis <br> | Lipases in H. utahensis <br> | ||
| − | ''' | + | |
| − | With defined EC number:''' | + | '''With defined EC number:''' |
3.1.4.46 Glycerophosphoryl diester phosphodiesterase <br> | 3.1.4.46 Glycerophosphoryl diester phosphodiesterase <br> | ||
| Line 7: | Line 7: | ||
Stop: 1166433 <br> | Stop: 1166433 <br> | ||
Amino acid sequence: <br> | Amino acid sequence: <br> | ||
| − | MFHVASSPSRPGSDYRGSSSAPPEKPLQGGDGRAGMRCIAHRGFAGLY | + | MFHVASSPSRPGSDYRGSSSAPPEKPLQGGDGRAGMRCIAHRGFAGLY |
| − | PENTLTAVRQAAAQADMIEVDVRRCASGELVVFHDETVDRLTDETGAVAE | + | PENTLTAVRQAAAQADMIEVDVRRCASGELVVFHDETVDRLTDETGAVAE |
| − | YTAAELADMDIDGSGEGVPELSAVLEAVPPDVGLDLELKESGLIEDVREA | + | YTAAELADMDIDGSGEGVPELSAVLEAVPPDVGLDLELKESGLIEDVREA |
| − | VKGADVDVWLSSFDTDVVARATETTDVPVVLIVDDASEAAIERAAGLGCA | + | VKGADVDVWLSSFDTDVVARATETTDVPVVLIVDDASEAAIERAAGLGCA |
| − | GIAPHWRLIDFALIERAHRSDLAVYAWPINDIDRAASLQKMDVDGIVAEE | + | GIAPHWRLIDFALIERAHRSDLAVYAWPINDIDRAASLQKMDVDGIVAEE |
PTACTGD <br> | PTACTGD <br> | ||
| Line 29: | Line 29: | ||
Esterase/lipase <br> | Esterase/lipase <br> | ||
| − | Start: 273678 (JGI); 273507 (RAST) | + | Start: 273678 (JGI); 273507 (RAST) <br> |
| − | Stop: 274619 | + | Stop: 274619 <br> |
| − | Amino acid sequence (RAST): | + | Amino acid sequence (RAST):<br> |
MGRLHVGVRLRLISSPVLVFHTNEGGLKQTGTHRTVRLLPTDPFTPRFCY | MGRLHVGVRLRLISSPVLVFHTNEGGLKQTGTHRTVRLLPTDPFTPRFCY | ||
RRFNLAPMRADEMNSQAQAVVDRQRRLGISPISDHDHRIMRLLQRVSTWF | RRFNLAPMRADEMNSQAQAVVDRQRRLGISPISDHDHRIMRLLQRVSTWF | ||
| Line 39: | Line 39: | ||
DDQASVRENDGIVLSVADLEWFRECYYDGDIHQRNPYADPANACDIGDVA | DDQASVRENDGIVLSVADLEWFRECYYDGDIHQRNPYADPANACDIGDVA | ||
PATVVTAGFDPIRDGGVAYAEQLDADGVDVTHRHYPDMIHGFATESSIDR | PATVVTAGFDPIRDGGVAYAEQLDADGVDVTHRHYPDMIHGFATESSIDR | ||
| − | AEVALRAVASDFAATVGDES | + | AEVALRAVASDFAATVGDES<br> |
| − | Lysophospholipase (JGI) | + | Lysophospholipase (JGI) <br> |
| − | Start: 422303 (JGI); 422234 (RAST) | + | Start: 422303 (JGI); 422234 (RAST)<br> |
| − | Stop: 423799 | + | Stop: 423799<br> |
| − | Amino acid sequence (JGI): | + | Amino acid sequence (JGI):<br> |
MRTRRRFLQTCIALSGASLAGCSEDDESTTPTSTTTTAVSTTASTPETDT | MRTRRRFLQTCIALSGASLAGCSEDDESTTPTSTTTTAVSTTASTPETDT | ||
ATPEPSESTATDEPTETTEPTETESIDPETLKETGRAFVNQLAAGEYAAI | ATPEPSESTATDEPTETTEPTETESIDPETLKETGRAFVNQLAAGEYAAI | ||
| Line 54: | Line 54: | ||
EYLFRLDGELSDEEATQLQAVEQTVERIRSLDISEDEIRFGLGGRPFWRT | EYLFRLDGELSDEEATQLQAVEQTVERIRSLDISEDEIRFGLGGRPFWRT | ||
VQEYDQVATAKELDVPLAIFQGERDYQVTVENGYNQWQQALGDRENVTFR | VQEYDQVATAKELDVPLAIFQGERDYQVTVENGYNQWQQALGDRENVTFR | ||
| − | SYADLNHLFMPGEGQPSPAEYFQPNNVSRAVVEDVASWVRRVTGEDNQ | + | SYADLNHLFMPGEGQPSPAEYFQPNNVSRAVVEDVASWVRRVTGEDNQ <br> |
| + | Esterase/lipase <br> | ||
| + | Start: 948286 <br> | ||
| + | Stop: 947264 <br> | ||
| + | Amino acid sequence: <br> | ||
| + | MSRSDSDSAEETTTDRAMSVERFQGVTYAERDAGDMQLDLFIPETEDPPL | ||
| + | VVYVHGGGWAYATRANAPDLERYAAEWGVAFASVSYRLAPIPDDSPFDPA | ||
| + | PDNPTPRDIFPAQIVDVKAAIRWLRANADQYGYNAADVAVWGASAGGHLA | ||
| + | ALAGTLEDISDLAGEVYSPDHVEKAVAPDHSGRVQAVVDWYGISDLRELG | ||
| + | GDGLESLLLGDTVSENPEKARLGSPITHVSETTPPFLVMHGEADDVVPFE | ||
| + | QTELFADALHQAGVDVTVHALVDLGHVFGADSERSAMDRLTTTPRPAQSV | ||
| + | TVRTHFEAGEADPGQSVTQQAGPAAIKHFLSETIGFSADP <br> | ||
| − | Start: | + | Esterase/lipase <br> |
| − | Stop: | + | Start: 2361204 <br> |
| − | Amino acid sequence: | + | Stop: 2360203 <br> |
| − | + | Amino acid sequence: <br> | |
| − | + | MSDPTIHQGIPYADRESGEMKLDLYVPERENPPLVVYVHGGGWIAATRSN | |
| − | + | IPDPAKYAAEWGCAIASVSYRLQEAPDGAGVEEMYDPDNPTPRGSFPDHF | |
| − | + | VDVKAAIRWLRAHAEDYGYDADQIAAWGSSAGGHLALLAGVVDDVTDLGD | |
| − | + | AFADEVEKTVAPAESGAVQAVISWYGATDFRLIEDMSDEAQDDDGLIPLL | |
| + | LGGPQSEHPERWAQASPVTHVTPESPPALLMHGRKDEVVAVEHSRQFFDA | ||
| + | LENAGVDAVFYELHDLNHVWVERVEEIEGERVAMDLLAADPTHAQSVYEA | ||
| + | THVEEGGSPDRLFGDLPPAGPEAILEFLERTIK <br> | ||
Revision as of 15:46, 6 November 2008
Lipases in H. utahensis
With defined EC number:
3.1.4.46 Glycerophosphoryl diester phosphodiesterase
Start: 1165666
Stop: 1166433
Amino acid sequence:
MFHVASSPSRPGSDYRGSSSAPPEKPLQGGDGRAGMRCIAHRGFAGLY
PENTLTAVRQAAAQADMIEVDVRRCASGELVVFHDETVDRLTDETGAVAE
YTAAELADMDIDGSGEGVPELSAVLEAVPPDVGLDLELKESGLIEDVREA
VKGADVDVWLSSFDTDVVARATETTDVPVVLIVDDASEAAIERAAGLGCA
GIAPHWRLIDFALIERAHRSDLAVYAWPINDIDRAASLQKMDVDGIVAEE
PTACTGD
Without EC number:
Esterase/lipase
Start: 13469
Stop: 14503
Amino acid sequence:
MTGPPTWTRRGVLTALLAAGAGCTTETDDSTTSTQEPSDPETPTDEPSER
STTSAPTPTETATTTEEPTATDEPTTEEPTTTAESMEEITDVAFRETPQR
TLRLDLYLPSTEESSPFVVYAHGGGWIGGDKGHRPMFDRLVEEGFAVADI
QYRLAQEKQYPAAVRDVVAAVKWVRANAGEYGIDSERGALAGYSAGAHLA
ALVAVAPDHENFQPDEFHPDVPVGVDALVGYSGPYDFTESGIGENPLVAA
FFGADAPTERLEEGSPVSHVEGDDPPALLIHGTDDNVVPYRSTTALADAY
RNAGVPVDVLTGDGAGHGMIDNDEWREETLPSQEQFLADHLLTQ
Esterase/lipase
Start: 273678 (JGI); 273507 (RAST)
Stop: 274619
Amino acid sequence (RAST):
MGRLHVGVRLRLISSPVLVFHTNEGGLKQTGTHRTVRLLPTDPFTPRFCY
RRFNLAPMRADEMNSQAQAVVDRQRRLGISPISDHDHRIMRLLQRVSTWF
GNRDPPAVGATTDGAIPGPEGDLPVRLYRPDGPGPYPTIVFFHGGGFVLG
SIGTHDWLCRQLTRETGAVVVSVDYRLAPEHPFPAAVEDAYAATQWAADN
PDRLASDGTLAVAGDSAGGNLAAVVALLARDRGEPAVDYQALLYPGIGIS
DDQASVRENDGIVLSVADLEWFRECYYDGDIHQRNPYADPANACDIGDVA
PATVVTAGFDPIRDGGVAYAEQLDADGVDVTHRHYPDMIHGFATESSIDR
AEVALRAVASDFAATVGDES
Lysophospholipase (JGI)
Start: 422303 (JGI); 422234 (RAST)
Stop: 423799
Amino acid sequence (JGI):
MRTRRRFLQTCIALSGASLAGCSEDDESTTPTSTTTTAVSTTASTPETDT
ATPEPSESTATDEPTETTEPTETESIDPETLKETGRAFVNQLAAGEYAAI
MDDYAFSEQFAAQVDAEQLESIWTTQTASLGQFVELAGAEYSRSDGYHVT
VVLARFTGGRQAVRLVFDDTATIVGLFFPASGGSYSPPEYVNQSAFEEVD
RTIAATETCSLPAKLTLPTGEESIPGVVLVHGSGPNDMDETLGPNKPFKD
LAWGLASRGVAVLRYDKRTHACDVDRAALTLDEKVTDDALTALGVLREHP
RIDPSRTVVVGHSIGAMTAPRIADRDGSVAGAVMLAGNARPLLDVIPEQQ
EYLFRLDGELSDEEATQLQAVEQTVERIRSLDISEDEIRFGLGGRPFWRT
VQEYDQVATAKELDVPLAIFQGERDYQVTVENGYNQWQQALGDRENVTFR
SYADLNHLFMPGEGQPSPAEYFQPNNVSRAVVEDVASWVRRVTGEDNQ
Esterase/lipase
Start: 948286
Stop: 947264
Amino acid sequence:
MSRSDSDSAEETTTDRAMSVERFQGVTYAERDAGDMQLDLFIPETEDPPL
VVYVHGGGWAYATRANAPDLERYAAEWGVAFASVSYRLAPIPDDSPFDPA
PDNPTPRDIFPAQIVDVKAAIRWLRANADQYGYNAADVAVWGASAGGHLA
ALAGTLEDISDLAGEVYSPDHVEKAVAPDHSGRVQAVVDWYGISDLRELG
GDGLESLLLGDTVSENPEKARLGSPITHVSETTPPFLVMHGEADDVVPFE
QTELFADALHQAGVDVTVHALVDLGHVFGADSERSAMDRLTTTPRPAQSV
TVRTHFEAGEADPGQSVTQQAGPAAIKHFLSETIGFSADP
Esterase/lipase
Start: 2361204
Stop: 2360203
Amino acid sequence:
MSDPTIHQGIPYADRESGEMKLDLYVPERENPPLVVYVHGGGWIAATRSN
IPDPAKYAAEWGCAIASVSYRLQEAPDGAGVEEMYDPDNPTPRGSFPDHF
VDVKAAIRWLRAHAEDYGYDADQIAAWGSSAGGHLALLAGVVDDVTDLGD
AFADEVEKTVAPAESGAVQAVISWYGATDFRLIEDMSDEAQDDDGLIPLL
LGGPQSEHPERWAQASPVTHVTPESPPALLMHGRKDEVVAVEHSRQFFDA
LENAGVDAVFYELHDLNHVWVERVEEIEGERVAMDLLAADPTHAQSVYEA
THVEEGGSPDRLFGDLPPAGPEAILEFLERTIK
