Difference between revisions of "LuxR/cI"

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'''Parallel Biobrick Assembly'''
== Parallel Biobrick Assembly ==
 
  
 
'''Objective 1''': pLux/cI + RBS + LuxS + RBS + Mnt + TT  (ampicillin resistance)
 
'''Objective 1''': pLux/cI + RBS + LuxS + RBS + Mnt + TT  (ampicillin resistance)
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| 1B || B0034 || RBS  || RX/SP || RX || 12bp || K091109 || LuxS Gene || RX/SP || RS || 516bp ||  ||
 
| 1B || B0034 || RBS  || RX/SP || RX || 12bp || K091109 || LuxS Gene || RX/SP || RS || 516bp ||  ||
 
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| 2 || B0034 || RBS || RX/SP || SP || 12bp || Product of 1B || LuxS + RBS || XP || || || ||
 
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| 3 || Product of 2 || RBS + LuxS + RBS || RX/SP || SP || || Product of 1A || Mnt Gene + TT || RX/SP || XP || || ||
 
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Revision as of 15:46, 2 July 2008

Parallel Biobrick Assembly

Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)

K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]

Step BBa_ Description Prefix/suffix Enzymes Size BBa_ Description Prefix/suffix Enzymes Size BBa_ Size
. Vector . . . . Insert . . . . Product .
1A B0015 Double Terminator RX/SP RX 129bp C0072 Mnt Gene RX/SP RS 288bp
1B B0034 RBS RX/SP RX 12bp K091109 LuxS Gene RX/SP RS 516bp
2 B0034 RBS RX/SP SP 12bp Product of 1B LuxS + RBS XP
3 Product of 2 RBS + LuxS + RBS RX/SP SP Product of 1A Mnt Gene + TT RX/SP XP


Objective 2: pBAD + RBS + LuxR + TT (kanamycin resistance)


Step BBa_ Description Prefix/suffix Enzymes Size BBa_ Description Prefix/suffix Enzymes Size BBa_ Size
. Vector . . . . Insert . . . . Product .
1